Coding

Part:BBa_K2272017:Design

Designed by: Eric Joshua Wang   Group: iGEM17_Virginia   (2017-10-22)


pMnSOD-HAO


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 480
    Illegal XhoI site found at 2044
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 496
    Illegal NgoMIV site found at 973
    Illegal NgoMIV site found at 977
    Illegal NgoMIV site found at 2745
    Illegal NgoMIV site found at 3135
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 400
    Illegal BsaI.rc site found at 448
    Illegal BsaI.rc site found at 1015
    Illegal BsaI.rc site found at 3175


Design Notes

The MnSOD promoter and the hydroxylamine oxidoreductase coding sequence was ligated with SCAR.


Source

https://parts.igem.org/Part:BBa_K2272001 https://parts.igem.org/Part:BBa_K2272002

References